How much bacteriostatic water do you add to a 5 mg Semax vial?

A common choice is 2 mL, giving a concentration of 2.5 mg/mL. The exact volume is up to you — more water makes a more dilute, easier-to-measure solution. Use the calculator above to match your target dose to a clean syringe mark.

How many units of Semax do I draw for a 250 mcg dose?

With a 5 mg vial in 2 mL of BAC water, a 250 mcg dose is 10 units on a U-100 insulin syringe.

Nootropic

Semax reconstitution calculator

Also known as N-acetyl Semax

Reconstitute Semax by adding bacteriostatic water to the vial, then draw your dose on a U-100 insulin syringe. The calculator is pre-filled with commonly-referenced vial sizes (5, 10 mg) and water volumes (2, 3 mL) — every field is yours to override. ACTH(4-10) analog; often used intranasally.

New to this? How to use the calculator

1Enter what's on your vial. Type the peptide amount (mg) printed on the vial and the mL of bacteriostatic water you mixed in. Not sure how much water to add? Switch to the Solve: water tab and it tells you.
2Enter your dose — mind the unit. Type your target dose and pick mg or mcg with the toggle. This matters: 1 mg = 1000 mcg. GLP-1s (semaglutide, tirzepatide) are usually mg; most peptides (BPC-157, ipamorelin) are mcg.
3Read “Draw to … units”. The big number is how far to pull the plunger on a U-100 insulin syringe. Match it to the fill line on the syringe picture. The concentration and doses per vial are shown so a wrong answer looks wrong.

Three ways to use it: Solve: units (you have a dose, find the draw), Solve: water (you want a clean mark like 10 units, find the water), and Solve: dose (you drew some units, find the dose).

Full step-by-step guide

I have a vial, water and a dose — how many units do I draw?

Peptide in vial (mg)BAC water added (mL)Target dose

Syringe size

Vial cost (optional)

Draw to

10 u

Concentration

2.5 mg/mL

Dose volume

0.1 mL

Doses / vial

Want this protocol tracked, scheduled and burned down by half-life?

Track this protocol

This tool computes volumes only — it does not recommend doses. Any pre-filled values are commonly-referenced examples to confirm with your provider. Always verify the concentration and draw against your own vial and syringe.

Semax concentration quick-reference

Vial	BAC water	Concentration	Units for 250 mcg
5 mg	2 mL	2.5 mg/mL	10 u
5 mg	3 mL	1.67 mg/mL	15 u
10 mg	2 mL	5 mg/mL	5 u
10 mg	3 mL	3.33 mg/mL	7.5 u

Example inputs for illustration. Confirm your actual dose with your provider.

Semax has a reported half-life of about 30 min. Track how much stays active between doses with a real pharmacokinetic burn-down.

Track Semax in PeptideBuddy

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<iframe src="https://www.peptidebuddy.ai/embed/recon/semax" width="100%" height="640" style="border:1px solid #243150;border-radius:16px" title="Semax reconstitution calculator"></iframe>

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