How much bacteriostatic water do you add to a 100 mg NAD+ vial?

A common choice is 3 mL, giving a concentration of 33.3 mg/mL. The exact volume is up to you — more water makes a more dilute, easier-to-measure solution. Use the calculator above to match your target dose to a clean syringe mark.

How many units of NAD+ do I draw for a 50 mg dose?

With a 100 mg vial in 3 mL of BAC water, a 50 mg dose is 150 units on a U-100 insulin syringe.

Metabolic

NAD+ reconstitution calculator

Also known as Nicotinamide adenine dinucleotide

Reconstitute NAD+ by adding bacteriostatic water to the vial, then draw your dose on a U-100 insulin syringe. The calculator is pre-filled with commonly-referenced vial sizes (100, 500 mg) and water volumes (3, 5, 10 mL) — every field is yours to override. Coenzyme used for cellular energy/longevity protocols.

New to this? How to use the calculator

1Enter what's on your vial. Type the peptide amount (mg) printed on the vial and the mL of bacteriostatic water you mixed in. Not sure how much water to add? Switch to the Solve: water tab and it tells you.
2Enter your dose — mind the unit. Type your target dose and pick mg or mcg with the toggle. This matters: 1 mg = 1000 mcg. GLP-1s (semaglutide, tirzepatide) are usually mg; most peptides (BPC-157, ipamorelin) are mcg.
3Read “Draw to … units”. The big number is how far to pull the plunger on a U-100 insulin syringe. Match it to the fill line on the syringe picture. The concentration and doses per vial are shown so a wrong answer looks wrong.

Three ways to use it: Solve: units (you have a dose, find the draw), Solve: water (you want a clean mark like 10 units, find the water), and Solve: dose (you drew some units, find the dose).

Full step-by-step guide

I have a vial, water and a dose — how many units do I draw?

Peptide in vial (mg)BAC water added (mL)Target dose

Syringe size

Vial cost (optional)

Draw to

150 u

Concentration

33.33 mg/mL

Dose volume

1.5 mL

Doses / vial

This dose needs 150 units, but a 0.5 mL syringe only holds 50. Use less BAC water (a higher concentration), split the dose, or pick a larger syringe.

Want this protocol tracked, scheduled and burned down by half-life?

Track this protocol

This tool computes volumes only — it does not recommend doses. Any pre-filled values are commonly-referenced examples to confirm with your provider. Always verify the concentration and draw against your own vial and syringe.

NAD+ concentration quick-reference

Vial	BAC water	Concentration	Units for 50 mg
100 mg	3 mL	33.3 mg/mL	150 u
100 mg	5 mL	20 mg/mL	250 u
100 mg	10 mL	10 mg/mL	500 u
500 mg	3 mL	167 mg/mL	30 u
500 mg	5 mL	100 mg/mL	50 u
500 mg	10 mL	50 mg/mL	100 u

Example inputs for illustration. Confirm your actual dose with your provider.

NAD+ has a reported half-life of about 1.0 h. Track how much stays active between doses with a real pharmacokinetic burn-down.

Track NAD+ in PeptideBuddy

Embed this calculator

Drop the NAD+ calculator into your forum post or blog:

<iframe src="https://www.peptidebuddy.ai/embed/recon/nad-plus" width="100%" height="640" style="border:1px solid #243150;border-radius:16px" title="NAD+ reconstitution calculator"></iframe>

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